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human unprocessed whole bone marrow aspirates  (Poietics Inc)

 
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    Structured Review

    Poietics Inc human unprocessed whole bone marrow aspirates
    Human Unprocessed Whole Bone Marrow Aspirates, supplied by Poietics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+unprocessed+whole+bone+marrow+aspirates/human+unprocessed+whole+bone+marrow+aspirates/us07674882-372-1-15
    Average 90 stars, based on 1 article reviews
    human unprocessed whole bone marrow aspirates - by Bioz Stars, 2026-10
    90/100 stars

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    Modification:

    Article Title: Emulsan–Alginate Beads for Protein Adsorption
    Article Snippet: Emulsan–alginate beads were prepared and challenged using bovine serum albumin (BSA) to assess adsorption in comparison to alginate beads.. BSA binding to the emulsan–alginate beads was improved over the alginate bead controls and protein adsorption was less sensitive to changes in ionic strength.. BSA adsorption between pH 8.5 and 5.3 in alginate beads was 2–3-times lower compared to the emulsan–alginate beads in the same pH range.

    Article Title: Silk biomaterials and methods of use thereof
    Article Snippet: .. Briefly, human unprocessed whole bone marrow aspirates were obtained from donors <25 years of age (Clonetic-Poietics, Walkersville, Md.), resuspended in Dulbecco Modified Eagle Medium (DMEM) supplement with 10% fetal bovine serum (FBS), 0.1 mM nonessential amino acids, 100 U/ml penicillin and 100 mg/L streptomycin (P/S), and 1 ng/ml basic fibroblast growth factor (bFGF) and plated at 8 μl aspirate/cm2 in tissue culture polystyrene; non-adherent hematopoietic cells were removed with the culture medium during medium exchange after 4 days. ..

    Article Title: Human bone marrow stromal cell and ligament fibroblast responses on RGD-modified silk fibers.
    Article Snippet: Adhesion, spreading, proliferation, and collagen matrix production of human bone marrow stromal cells (BMSCs) on an RGD-modified silk matrix was studied.. Anterior cruciate ligament fibroblasts (ACLFs) were used as a control cell source.. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the modified silk matrices support improved BMSC and ACLF attachment and show higher cell density over 14 days in culture when compared with the non-RGD-modified matrices.

    Tissue:

    Article Title: Silk biomaterials and methods of use thereof
    Article Snippet: .. Briefly, human unprocessed whole bone marrow aspirates were obtained from donors <25 years of age (Clonetic-Poietics, Walkersville, Md.), resuspended in Dulbecco Modified Eagle Medium (DMEM) supplement with 10% fetal bovine serum (FBS), 0.1 mM nonessential amino acids, 100 U/ml penicillin and 100 mg/L streptomycin (P/S), and 1 ng/ml basic fibroblast growth factor (bFGF) and plated at 8 μl aspirate/cm2 in tissue culture polystyrene; non-adherent hematopoietic cells were removed with the culture medium during medium exchange after 4 days. ..

    Isolation:

    Article Title: Human bone marrow stromal cell and ligament fibroblast responses on RGD-modified silk fibers.
    Article Snippet: Adhesion, spreading, proliferation, and collagen matrix production of human bone marrow stromal cells (BMSCs) on an RGD-modified silk matrix was studied.. Anterior cruciate ligament fibroblasts (ACLFs) were used as a control cell source.. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the modified silk matrices support improved BMSC and ACLF attachment and show higher cell density over 14 days in culture when compared with the non-RGD-modified matrices.

    Cell Culture:

    Article Title: Human bone marrow stromal cell and ligament fibroblast responses on RGD-modified silk fibers.
    Article Snippet: Adhesion, spreading, proliferation, and collagen matrix production of human bone marrow stromal cells (BMSCs) on an RGD-modified silk matrix was studied.. Anterior cruciate ligament fibroblasts (ACLFs) were used as a control cell source.. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the modified silk matrices support improved BMSC and ACLF attachment and show higher cell density over 14 days in culture when compared with the non-RGD-modified matrices.

    other:

    Article Title: Cell differentiation by mechanical stress.
    Article Snippet: Growth factors, hormones, and other regulatory molecules are traditionally required in tissue engineering studies to direct the differentiation of progenitor cells along specific lineages.. We demonstrate that mechanical stimulation in vitro, without ligament-selective exogenous growth and differentiation factors, induces the differentiation of mesenchymal progenitor cells from the bone marrow into a ligament cell lineage in preference to alternative paths (i.e., bone or cartilage cell lineages).. A bioreactor was designed to permit the controlled application of ligament-like multidimensional mechanical strains (translational and rotational strain) to the undifferentiated cells embedded in a collagen gel.



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